2-Amino-2-(hydroxymethyl)-1,3-propanediol (CAS 77-86-1) — Citrus N/A Note Fragrance Ingredient

Citrus · Floral

2-Amino-2-(hydroxymethyl)-1,3-propanediol

CAS 77-86-1

Origin
synthetic
Note
N/A
IFRA
Use with awareness
Data as of: Apr 2026

What Is 2-Amino-2-(hydroxymethyl)-1,3-propanediol?

2-Amino-2-(hydroxymethyl)-1,3-propanediol, commonly known as tris(hydroxymethyl)aminomethane or TRIS, is a synthetic compound primarily used in biochemistry and molecular biology. It’s found in buffer solutions for DNA/RNA extraction and protein purification rather than in consumer products. While not a fragrance ingredient, its chemical properties make it valuable for stabilizing pH-sensitive compounds in laboratories and pharmaceutical formulations.

Safety Profile

USE WITH AWARENESS
Generally safeUse with awarenessProfessional use
Low toxicity in controlled applications
May cause irritation at high concentrations
CAS
77-86-1
Formula
Mixture
MW
Variable
Odor Family
Citrus · Floral
Layer 1 · Enthusiast

What Does 2-Amino-2-(hydroxymethyl)-1,3-propanediol Smell Like?

This compound is essentially odorless in pure form, with no discernible fragrance characteristics. As a buffering agent rather than a perfumery material, it lacks the volatile organic compounds that typically contribute to scent profiles. In concentrated solutions, some users report a faint, non-descript chemical odor similar to other amine-containing compounds, but this is not considered a meaningful olfactory property.

Scent Profile

In Famous Fragrances

Fragrance associations may not reflect actual formulations.

Layer 2

2D Molecular Structure

Tromethamine

SMILES: NC(CO)(CO)CO

Chemistry, Properties & Perfumer Guide

The Chemistry

2-Amino-2-(hydroxymethyl)-1,3-propanediol is a tertiary amine with three hydroxyl groups, giving it excellent water solubility and buffering capacity between pH 7-9. The compound is synthesized through the reaction of nitromethane with formaldehyde under basic conditions, followed by hydrogenation. Its molecular structure features a central carbon atom bonded to both an amino group and three hydroxymethyl groups, creating a versatile scaffold for chemical modifications.

Physical & Chemical Properties

Melting Point171-172 °C
Solubility550 g/L in water (20°C)

Perfumer Guide

Note Position
N/A
Volatility
N/A
Blending
N/A
ApplicationTypical %RangeNotes
Laboratory Use10-100 mMUp to 1MBuffer solutions
Pharmaceuticals0.1-1%Up to 5%Stabilizing agent

Classic Accords

Tip: Not applicable for perfumery use.

Alternatives & Comparisons

1
HEPES CAS 7365-45-9

Used as alternative biological buffer with different pH range (7.2-8.2) and lower temperature sensitivity.

2
Phosphate Buffered Saline CAS N/A

Classic physiological buffer system for applications requiring neutral pH conditions.

Layer 3

Safety, Regulatory & Sustainability

⚠ Regulatory Disclaimer

General reference only. Consult current IFRA Standards Library before formulating.

IFRA Status

Not regulated by IFRA as it is not a fragrance ingredient.

GHS Classification

H315 Skin irritation H319 Eye irritation H335 May cause respiratory irritation

RIFM Assessment

Not evaluated by RIFM as it falls outside fragrance material scope.

Sustainability

As a synthetic compound produced through controlled chemical processes, TRIS has minimal environmental impact when properly disposed. Its production doesn’t rely on scarce natural resources, and its high water solubility reduces bioaccumulation potential. However, energy-intensive manufacturing and potential aquatic toxicity at high concentrations warrant responsible handling.

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References

  1. Good, N.E. et al. (1966). Hydrogen Ion Buffers for Biological Research. Biochemistry. DOI:10.1021/bi00866a011
  2. Sigma-Aldrich TRIS Safety Data Sheet SDS 252859

Data: PubChem (NIH), PubMed, RIFM, IFRA. Last reviewed: Apr 2026.

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Ingredient Data Sheet

CAS 77-86-1

Physical Properties

Molecular Weight121.14 g/mol🔬 PubChem
LogP (Octanol-Water)-2.9🔬 PubChem
Boiling Point219 °C🔬 EPA CompTox
Vapor Pressure0 mmHg @ 25°C📊 OPERA
Flash Point169.7 °C🔬 EPA CompTox
log Kp (skin permeability)-5.498💻 Calculated
SMILESC(C(CO)(CO)N)O🔬 PubChem

Volatility & Performance

Fragrance NoteBase💻 Calculated
Volatility ClassVery slow💻 Calculated
Persistence Score1.7 / 5💻 Calculated

Odor & Flavor

Primary Descriptorscitrusfloral• leffingwell
Functional Groupsalcoholamine💻 RDKit
Data Sources & Attribution
Physical data: PubChem (NIH/NLM), U.S. EPA CompTox Dashboard, EPA OPERA models, RDKit. Odor & flavor: Arctander (Perfume & Flavor Chemicals), Fenaroli's Handbook of Flavor Ingredients, Leffingwell. Thresholds: van Gemert (Compilations of Odour Threshold Values). Regulatory: IFRA Standards 51st, FEMA GRAS. Trade names: Surburg (Common Fragrance & Flavor Materials). All data compiled and cross-referenced for perfumertools.com.

Physicochemical Properties

DTXSID: DTXSID2023723

Physical Properties

Molecular Weight 121.136 g/mol🔬 EPA CompTox
Density 1.32 g/cm^3🔬 EPA CTX
Boiling Point 288 °C🔬 EPA CTX
Melting Point 171.111 °C🔬 EPA CTX
Flash Point 166.457 °C📊 OPERA
Refractive Index 1.544 Dimensionless📊 OPERA
Molar Volume 90.806 cm^3/mol📊 OPERA

Partition & Solubility

LogP (Octanol-Water) 0.005 Log10 unitless🔬 EPA CTX
LogD (pH 5.5) -5.043 Log10 unitless📊 OPERA
LogD (pH 7.4) -3.649 Log10 unitless📊 OPERA
LogKoa (Octanol-Air) 7.18 Log10 unitless📊 OPERA
Water Solubility 5.011 mol/L🔬 EPA CTX
Henry's Law Constant 0 atm-m3/mole📊 OPERA

Transport Properties

Vapor Pressure 0 mmHg🔬 EPA CTX
Viscosity 71.003 cP📊 OPERA
Surface Tension 56.292 dyn/cm📊 OPERA

Molecular Descriptors

Topological Polar Surface Area 86.71 Ų💻 Computed
H-Bond Donors 4 count💻 Computed
H-Bond Acceptors 4 count💻 Computed
Rotatable Bonds 3 count💻 Computed
Aromatic Rings 0 count💻 Computed
Molar Refractivity 28.686 cm^3/mol📊 OPERA
Polarizability 11.372 Å^3📊 OPERA

Data Sources:

🔬 EPA Experimental data from U.S. EPA CompTox Chemicals Dashboard & CTX APIs. 📊 OPERA Predicted using EPA's OPERA QSAR models. 💻 Computed Calculated from SMILES using RDKit.

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