Glycyrrhizin, ammoniated (Glycyrrhiza spp.) (CAS 53956-04-0) — Sweet Base Note Fragrance Ingredient

Sweet · Balsamic

Glycyrrhizin, ammoniated (Glycyrrhiza spp.)

CAS 53956-04-0

Origin
synthetic
Note
Base
IFRA
Use with awareness
Data as of: Apr 2026

What Is Glycyrrhizin, ammoniated (Glycyrrhiza spp.)?

Ammoniated glycyrrhizin is a sweetening compound derived from licorice root (Glycyrrhiza spp.). It’s commonly found in confectionery, tobacco products, and some beverages. This ingredient provides a distinctive sweet flavor that’s 50 times more potent than sugar, making it valuable for flavor modulation where intense sweetness is desired without bulk.

Safety Profile

USE WITH AWARENESS
Generally safeUse with awarenessProfessional use
Approved as food additive in many countries
May affect blood pressure in sensitive individuals
CAS
53956-04-0
Formula
Mixture
MW
Variable
Odor Family
Sweet · Balsamic
Layer 1 · Enthusiast

What Does Glycyrrhizin, ammoniated (Glycyrrhiza spp.) Smell Like?

While primarily a flavorant, ammoniated glycyrrhizin has subtle olfactory characteristics. It imparts a sweet, woody-anisic aroma reminiscent of dried licorice sticks with a faint ammoniacal nuance. The scent profile is persistent but not volatile, acting more as a background sweetener than a top note. Its olfactory effect is most noticeable in tobacco and leather accords where it provides a rounded sweetness that tempers harsh edges.

Scent Profile
Layer 2

2D Molecular Structure

Ammonium glycyrrhizate

SMILES: [NH4+].CC1(C)[C@H](CC[C@@]2(C)[C@H]1CC[C@]1(C)[C@@H]2C(=O)C=C2[C@@H]3C[C@](C)(CC[C@]3(C)CC[C@@]12C)C(O)=O)O[C@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O[C@@H]1O[C@@H]([C@@H](O)[C@H](O)[C@H]1O)C([O-])=O)C(O)=O

Chemistry, Properties & Perfumer Guide

The Chemistry

Ammoniated glycyrrhizin is the ammonium salt of glycyrrhizic acid, a triterpenoid saponin glycoside. The compound is typically produced by alkaline extraction from licorice root followed by ammoniation. Chemically, it consists of a glycyrrhetinic acid moiety with two glucuronic acid units. The ammoniation process increases water solubility while maintaining the characteristic sweet taste. Unlike its parent compound, it doesn’t exhibit the mineralocorticoid effects associated with glycyrrhizic acid.

Physical & Chemical Properties

AppearanceYellowish-brown powder
SolubilityWater-soluble

Perfumer Guide

Note Position
Base
Volatility
Very low
Blending
Specialized
ApplicationTypical %RangeNotes
Tobacco Flavors0.1-0.5%Up to 1%Sweetness enhancer
Leather Accords0.01-0.1%Trace amountsRoundness modifier

Classic Accords

Tip: Use sparingly in alcohol-based perfumes as it may precipitate.

Alternatives & Comparisons

1
Glycyrrhetinic acid CAS 471-53-4

The aglycone form with less sweetness but similar olfactory properties, useful when water solubility isn’t required.

2
Maltol CAS 118-71-8

Provides sweet caramelic notes without the licorice character, more volatile and versatile in compositions.

Layer 3

Safety, Regulatory & Sustainability

⚠ Regulatory Disclaimer

General reference only. Consult current IFRA Standards Library before formulating.

IFRA Status

Not restricted by IFRA, but use in fragrances is limited due to low volatility.

RIFM Assessment

Not currently evaluated by RIFM due to limited fragrance applications.

Sustainability

Licorice is a drought-resistant crop requiring minimal inputs, though overharvesting of wild plants is a concern. Most commercial material now comes from cultivated sources. Synthetic production routes exist but are not economically competitive with plant extraction. The ammoniation process is low-waste and uses common industrial chemicals.

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References

  1. European Food Safety Authority. (2010). Scientific Opinion on Glycyrrhizinic acid and its ammonium salt. EFSA Journal. EFSA Journal 2010
  2. Kitagawa, I. (2002). Licorice root. A natural sweetener and an important ingredient in Chinese medicine. Pure Appl. Chem. PAC 2002

Data: PubChem (NIH), PubMed, RIFM, IFRA. Last reviewed: Apr 2026.

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Perfumer’s Notes

FEMA #: 2528  |  IOFI #: Natural

Licorice (glycyrrhiza) root is the dried and ground rhizome and root portions of Glycyrrhiza glabra or other species of glycyrrhiza. Ammoniated glycyrrhizin is prepared from the water extract of licorice root by acid precipitation, followed by neutralization with dilute ammonia. Monoammonium glycyrrhizinate (C42H62O16NH45H2O) is prepared from ammoniated glycyrrhizin by solvent extraction and separation techniques.

MW: 840.0

Ingredient Data Sheet

CAS 53956-04-0

Physical Properties

Molecular Weight840 g/mol🔬 PubChem
Boiling Point378 °C🔬 EPA CompTox
Vapor Pressure0 mmHg @ 25°C📊 OPERA
SMILESCC1(C2CCC3(C(C2(CCC1OC4C(C(C(C(O4)C(=O)O)O)O)OC5C(C(C(C(O5)C(=O)O)O)O)O)C)C(=O)C=C6C3(CCC7(C6CC(CC7)(C)C(=O)O)C)C)C)C.N🔬 PubChem

Volatility & Performance

Fragrance NoteBase💻 Calculated

Odor & Flavor

Functional Groupsketonealcoholetheralkene💻 RDKit
“Very faint, sweet caramellic-rootlike odor.”📖 Arctander
Licorice (glycyrrhiza) root is the dried and ground rhizome and root portions of Glycyrrhiza glabra or other species of glycyrrhiza. Ammoniated glycyrrhizin is prepared from the water extract of licorice root by acid precipitation, followed by neutralization with dilute ammonia. Monoammonium glycyrrhizinate (C42H62O16NH45H2O) is prepared from ammoniated glycyrrhizin by solvent extraction and separation techniques.📖 Fenaroli

Flavor Notes (Arctander)

“Intensely sweet taste. In higher concentrations producing a slightly burning sensation in the back of the mouth and in the throat, shortly after the sweet taste. In extreme dilution there is only a sweet taste. Glycyrrhizin (ammoniated) as such is not widely used in flavors, but the impure Glycyrr- ”📖 Arctander

Regulatory Status

FEMA NumberFEMA 2528⚖️ FEMA GRAS
GRAS StatusGenerally Recognized as Safe⚖️ FEMA GRAS
IOFI ClassificationNatural📖 Fenaroli
Data Sources & Attribution
Physical data: PubChem (NIH/NLM), U.S. EPA CompTox Dashboard, EPA OPERA models, RDKit. Odor & flavor: Arctander (Perfume & Flavor Chemicals), Fenaroli's Handbook of Flavor Ingredients, Leffingwell. Thresholds: van Gemert (Compilations of Odour Threshold Values). Regulatory: IFRA Standards 51st, FEMA GRAS. Trade names: Surburg (Common Fragrance & Flavor Materials). All data compiled and cross-referenced for perfumertools.com.

Physicochemical Properties

DTXSID: DTXSID2041204

Physical Properties

Molecular Weight 839.973 g/mol🔬 EPA CompTox
Density 0.801 g/cm^3🔬 EPA CTX
Boiling Point 378 °C📊 OPERA
Melting Point 209 °C🔬 EPA CTX

Partition & Solubility

LogP (Octanol-Water) 1.86 Log10 unitless📊 OPERA
LogD (pH 5.5) 0.84 Log10 unitless📊 OPERA
LogD (pH 7.4) 1.81 Log10 unitless📊 OPERA
LogKoa (Octanol-Air) 9.59 Log10 unitless📊 OPERA
Water Solubility 0.003 mol/L📊 OPERA
Henry's Law Constant 0 atm-m3/mole📊 OPERA

Transport Properties

Vapor Pressure 0 mmHg📊 OPERA

Molecular Descriptors

Topological Polar Surface Area 306.37 Ų💻 Computed
H-Bond Donors 8 count💻 Computed
H-Bond Acceptors 14 count💻 Computed
Rotatable Bonds 7 count💻 Computed
Aromatic Rings 0 count💻 Computed
Molar Refractivity 204.46 cm^3/mol💻 Computed

Data Sources:

🔬 EPA Experimental data from U.S. EPA CompTox Chemicals Dashboard & CTX APIs. 📊 OPERA Predicted using EPA's OPERA QSAR models. 💻 Computed Calculated from SMILES using RDKit.

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